Oil Palm Bulletin No. 77 (November 2018) p8-13

Profiling of Metabolites Present in Ethyl Acetate Fractionation of Trichoderma virens 7b

Lee Pei Lee Angel*, Shamala Sundram*, Bonnie Tay Yen Ping*, Mohd Termizi Yusof** and Intan Safinar Ismail**

Endophytic Trichoderma virens 7b isolated from oil palm roots has been extensively studied to determine its potential against the oil palm pathogen, Ganoderma. The activities of its nonpolar metabolites were previously reported to show the highest inhibition on G. boninense PER71 growth compared to other extracts. Nevertheless, it is also important to understand the activity of its semipolar metabolites towards G. boninense. Therefore, this study aims to reveal the list of metabolites in T. virens 7b extracted using ethyl acetate and the effect of extracts on G. boninense PER71. The ethyl acetate extract led to a significant decrease in the growth rate of G. boninense PER71 from 6.7 mm day-1 to 2.9 mm day-1. This resulted in a percentage inhibition of radial growth (PIRG) of 59.63% ± 9.13. Coiling and clumping of the G. boninense’s hyphae observed by scanning electron microscope subjected to ethyl acetate extract explain the growth inhibition. The metabolite profiles showed the presence of potential antifungal compounds which may contribute to the inhibition. These include succinimide and isolongifolene, 4,5,9,10-dehydro.

Trichoderma virens 7b endofitik yang dipencil daripada akar pokok sawit telah dikaji untuk menentukan potensinya dalam menentang patogen sawit, Ganoderma. Aktiviti metabolit bukan polar yang dilaporkan sebelum ini telah menunjukkan perencatan pertumbuhan paling tinggi terhadap G. boninense PER71 berbanding ekstrak lain. Walau bagaimanapun, aktiviti metabolit semi-polar terhadap G. boninense adalah penting untuk dikaji. Oleh itu, kajian ini bertujuan untuk mengetahui profil metabolit dalam T. virens 7b yang diekstrak menggunakan etil asetat dan kesan ekstrak terhadap G. boninense PER71. Ekstrak etil asetat berjaya mengurangkan kadar pertumbuhan G. boninense PER71 dari 6.7 mm hari-1 kepada 2.9 mm hari-1. Hasilnya, peratus perencatan pertumbuhan G. boninense PER71 adalah sebanyak 59.63% ± 9.13. Cerapan mikroskop elektron imbasan (SEM) menunjukkan bahawa ekstrak etil asetat telah menyebabkan penggelungan dan pembentukan ketulan hifa G. boninense PER71, menjelaskan perencatan pertumbuhan G. boninense PER71. Profil metabolit menunjukkan kehadiran sebatian antifungal iaitu succinimide dan isolongifolene, 4,5,9,10-dehydro yang boleh menyumbang kepada perencatan G. boninense PER71.


Keywords: , , ,

Author information: