TS No. 135: Transient Gene Expression System Utilising Oil Palm Protoplasts

Aprotoplast is a single cell without cell wall and only has plasma membrane to protect the cell. This property helps the protoplast to easily take up foreign macromolecules such as DNA and has been used since 1974 for transient and stable transformation of many plant species. Protoplast transient gene expression system is well-known as an easy, rapid, safe and efficient system, particularly via polyethylene […]

TS No. 134: Genomsawit Website

The goal of the Malaysian Oil Palm Genome Programme (MyOPGP) is to produce gold standard references of the E. guineensis (EG) and E. oleifera (EO) genomes, which have a genome size of ~1.8 Gb. The sequences of both of these species, EG and EO and the shell gene were recently published in two back-to-back landmark publications in the prestigious journal – Nature (Singh et […]

TS No. 133: Makmal Sitogenetik MPOB

Makmal Sitogenetik MPOB has transferred several services as follows: PORIM TT No. 49 (1998). Chromosome Painting in Oil Palm Hybrids  Genomic in situ hybridisation (GISH) method was developed to distinguish between oleifera and guineensis chromosomes. Hence, this is a useful method for oil palm breeders to assess the genome composition of backcrosses and oil palm hybrids. MPOB TS No. 18 (2007). Flow […]

TS No. 132: Determination of Nutrients and H eavy Metals in Environmental Samples using Inductively Coupled Plasma Spectrometer (ICD-MS)

Interest in eco-friendly and sustainable agricultural practices using biological approach have increased rapidly with considerable success. However, extensive use of chemical fertilisers has led to environmental problems such as soil degradation, water pollution and emission of greenhouse gases. Environmental pollution has increased rapidly in the recent years, especially soil and groundwater contamination. Hence, it is vital to determine the concentration of contaminant rapidly and precisely, in […]

TS No. 131: Fungal Identification System 3 (FIDS 3)

Fungi play important roles in soil structure, soil fertility and soil-plant interaction. Therefore, it is crucial to identify them. The FIDS 1 is a fungal identification system using ITS region which identifies a limited range of fungal species whereas, the FIDS 2 identified the fungal through LSU fragments with broader range of fungal species and the system is suitable for large number of samples. […]