TT No. 299: Determination of Chlorpyrifos in Edible Oil (Liquid- Liquid Extraction Method)

The method involves the extraction of chlorpyrifos with hexane acetonitrile. Chlorpyrifos is preferentially partitioned into the polar acetonitrile layer while the bulk of the lipids remains solubilized in the non-polar hexane. The acetonitrile extract are subjected to clean-up procedure through a silicic acid column. The chlorpyrifos is analysed by gas chromatography equipped with an electron capture detector (GC-µECD)

Main Research: Halimah Muhamad